Recombinant Human VEGF165 Protein (HEK293, C-His) is a powerful growth and angiogenic cytokine. It promotes the proliferation and survival of endothelial cells, promotes angiogenesis and vascular permeability. VEGF is expressed in vascularized tissues and plays an important role in normal and pathological angiogenesis.
Measured by human umbilical vein endothelial cells (HUVEC) proliferation assay, the ED50 for this effect is ≤20 ng/mL.
DNA sequence encoding human VEGF165 (NP_001165097.1) is expressed with a His tag at the C terminus.
The recombinant human VEGF165 protein contains 193 amino acids and has a predicted theoretical molecular weight of 22.3 kD.
Endotoxin <0.1EU/μg
Cell Death Dis. 2023 Mar 11;14(3):194.
USP22 upregulates ZEB1-mediated VEGFA transcription in hepatocellular carcinoma
Recombinant Human VEGF165 Protein (HEK293, C-His) purchased from AbMole
Sci Rep. 2019 Nov 28;9(1):17745.
In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis
Recombinant Human VEGF165 Protein (HEK293, C-His) purchased from AbMole
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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| Recombinant Human DPP4/CD26 (Mammalian, C-6His)
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