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Recombinant Human VEGF165 Protein (HEK293, C-His)

Cat. No. M9413
Recombinant Human VEGF165 Protein (HEK293, C-His) Structure
Size Price Availability Quantity
50ug USD 540  USD540 In stock
100ug USD 880  USD880 In stock
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Quality Control & Documentation
  • Purity: >90%, Endotoxin <0.1EU/ug
  • COA
  • MSDS
Biological Activity

Recombinant Human VEGF165 Protein (HEK293, C-His) is a powerful growth and angiogenic cytokine. It promotes the proliferation and survival of endothelial cells, promotes angiogenesis and vascular permeability. VEGF is expressed in vascularized tissues and plays an important role in normal and pathological angiogenesis.

Measured by human umbilical vein endothelial cells (HUVEC) proliferation assay, the ED50 for this effect is ≤20 ng/mL.

DNA sequence encoding human VEGF165 (NP_001165097.1) is expressed with a His tag at the C terminus.

The recombinant human VEGF165 protein contains 193 amino acids and has a predicted theoretical molecular weight of 22.3 kD.

Endotoxin <0.1EU/μg

Product Citations
Chemical Information
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Robin M H Rumney, et al. Sci Rep. In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis

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