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Recombinant Human UBE2D1 (E.coli, N-GST)

Cat. No. M22462
Recombinant Human UBE2D1 (E.coli, N-GST) Structure
Synonym:

Ubiquitin-conjugating Enzyme E2 D1

Size Price Availability Quantity
10ug USD 58  USD58 In stock
50ug USD 150  USD150 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 1 EU/µg
  • COA
  • MSDS
Biological Activity

Recombinant Human UBE2D1 is produced by E.coli expression system and the target gene encoding Met1-Met147 is expressed with a GST tag at the N-terminus. Ubiquitin-conjugating enzyme E2 D1(UBE2D1)belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.


Accession: P51668

APMol Mass: 40 KDa, reducing conditions

Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.

Chemical Information
Storage Store at ≤-70°C, stable for 6 months after receipt
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Matthew E R Maitland, et al. Sci Rep. The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation

[2] Christine K Schmidt, et al. Nat Cell Biol. Systematic E2 screening reveals a UBE2D-RNF138-CtIP axis promoting DNA repair

[3] Jeong-Hoon Kim, et al. BMB Rep. Synergistic effect of two E2 ubiquitin conjugating enzymes in SCF(hFBH1) catalyzed polyubiquitination

[4] So Maezawa, et al. PLoS One. Ubiquitylation of terminal deoxynucleotidyltransferase inhibits its activity

[5] Alexander Espinosa, et al. Exp Cell Res. The autoantigen Ro52 is an E3 ligase resident in the cytoplasm but enters the nucleus upon cellular exposure to nitric oxide

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Keywords: Recombinant Human UBE2D1 (E.coli, N-GST), Ubiquitin-conjugating Enzyme E2 D1 supplier, Recombinant Proteins, inhibitors, activators


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