Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Thrombopoietin (TPO) produced by the liver and kidney which regulates the production of platelets.
Recombinant Human Thrombopoietin is produced by Mammalian expression system and expressed the target gene encoding Ser22-Gly353 with a 6His tag at the N-terminus, 6His tag at the C-terminus.
Accession: P40225
Endotoxin < 0.01 EU/µg
Apparent Molecular Weight: 70-90 KDa, reducing conditions
Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
| Form | Lyophilized powder |
| Solubility (25°C) | Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[4] Ian S Hitchcock et al. Br J Haematol. Thrombopoietin from beginning to end
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