Recombinant Human TRAIL/Apo2L Protein is a cytotoxic protein that can activate the rapid apoptosis of tumor cells, but does not work in normal cells. TRAIL-induced apoptosis is achieved by binding to two death signal receptors DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic "death domain" that activates the cell's apoptosis mechanism.
Measured by the cell growth inhibitory assay using RPMI-8226 cells, the ED50 is < 40.0 ng/ml, corresponding to a specific activity of > 2.5 × 104 units/mg.
Recombinant Human TRAIL/Apo2L expressed the target gene encoding Val114-Gly281.
Accession: P50591
Endotoxin < 0.2 EU/μg
Apparent Molecular Weight: ~19.6 kDa, reducing conditions
Lyophilized after extensive dialysis against PBS.
| Form | Lyophilized powder |
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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