Free shipping on all orders over $ 500

Recombinant Human S100A11 (E.coli )

Cat. No. M21526
Recombinant Human S100A11 (E.coli ) Structure
Synonym:

Protein S100-A11; Calgizzarin; Metastatic lymph node gene 70 protein; MLN 70

Size Price Availability Quantity
10ug USD 200  USD200 In stock
50ug USD 510  USD510 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
  • Purity: >95%, Endotoxin<1 EU/µg
  • COA
  • MSDS
Biological Activity

Species: Human
Expression system: E.coli
Purity: > 95% as determined by reduced SDS-PAGE
Endotoxin: LAL test measured less than 0.1 ng/µg (1EU/µg)
Molecular weight: 11.7 KDa
Storage and stability: The freeze-dried protein should be stored at ≤-20°C for one year after receiving. The recombinant protein solution can be stored at 2-8℃ for 2-7 days. The bisector of the recombinant sample was stable at ≤-20°C for 3 months.



Bioactivity: S100A11 is a member of the calcium-binding protein S100 family. Human S100A11 contains two EF hand motifs, 82% of which share the same amino acid sequence as mouse and rat S100A11. It forms a covalent homodimer and a disulfide tetramer under the action of transglutamine. S100A11 is secreted by keratinocytes and can be cross-linked into the keratinocyte envelope of the skin. Dimerization enhances its ability to signal keratinocytes via RAGE, induce EGF family protein production, and induce cell proliferation. Dimerization also causes S100A11 to bind to RAGE on chondrocytes, leading to chondrocyte hypertrophy and catabolism of cartilage matrix. In addition, S100A11 is found in the cytoplasm and is phosphorylated and transferred to the nucleus in response to DNA damage, RELM alpha exposure, or elevated extracellular calcium concentrations. Calcium also promotes the binding of S100A11 to S100B and to annexins A1, A2 and A6. The S100A11-Annexin A2 complexes are recruited to sites of plasma membrane damage where they promote membrane repair of migrating cancer cells. S100A11 is upregulated in various cancers, supporting tumor cell proliferation, invasion and migration. In addition, S100A11 is produced in the ovary and acts on cumulus cells to inhibit oocyte fertilization.

Chemical Information
Solubility (25°C) Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Mi Cheng, et al. Curr Med Sci. Inhibitory Effect of S100A11 on Airway Smooth Muscle Contraction and Airway Hyperresponsiveness

[2] Linqiang Zhang, et al. Cell Mol Gastroenterol Hepatol. S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis

[3] Franziska Foertsch, et al. FEBS J. S100A11 is involved in the regulation of the stability of cell cycle regulator p21(CIP1/WAF1) in human keratinocyte HaCaT cells

[4] Nicole M Marlatt, et al. Protein Expr Purif. Codon optimization for enhanced Escherichia coli expression of human S100A11 and S100A1 proteins

[5] H Inada, et al. Biochem Biophys Res Commun. Human S100A11 exhibits differential steady-state RNA levels in various tissues and a distinct subcellular localization

Related Recombinant Proteins Products
Recombinant Human IFN-α 2b Protein (E. coli)

IFN-α 2b (Interferon-Alpha 2b) [Cys24-Glu188 (Lys46Arg)] produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections.

Recombinant Rat IL-2 (Mammalian, C-6His)

Interleukin-2 (IL-2) is a O-glycosylated four α-helix bundle cytokine that has potent stimulatory activity for antigenactivated T cells. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.

Recombinant Human VCAM-1 Protein (Mammalian, C-Fc)

VCAM-1 is a single-pass type I membrane protein, and mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate.

Recombinant Mouse VCAM-1 Protein (Mammalian, C-Fc)

Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface protein belonging to the immunoglobulin superfamily. Mouse VCAM-1 is Important in cell-cell recognition. it appears to function in leukocyte-endothelial cell adhesion, and interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction.

Recombinant Human VCAM-1 Protein (Mammalian, C-6His)

VCAM-1 is a single-pass type I membrane protein, and mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate.

  Catalog
Abmole Inhibitor Catalog




Keywords: Recombinant Human S100A11 (E.coli ), Protein S100-A11; Calgizzarin; Metastatic lymph node gene 70 protein; MLN 70 supplier, Recombinant Proteins, inhibitors, activators


Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.