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Recombinant Human RANKL (E.coli)

Cat. No. M11464
Recombinant Human RANKL (E.coli) Structure
Synonym:

CD254

Size Price Availability Quantity
10 μg USD 290  USD290 In stock
50 μg USD 775  USD775 In stock
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Quality Control
  • Current batch:
  • Purity >90%, Endotoxin < 0.1 ng/ug (1 EU/ug)
  • COA
  • MSDS
Biological Activity

Recombinant Human RANKL is a type II membrane protein of the tumor necrosis factor (TNF) superfamily, and affects the immune system and control bone regeneration and remodeling. RANKL is the ligand of nuclear factor (NF)-κB (RANK). When RANKL binds to RANK, it will undergo trimerization and then bind to an adaptor molecule TNF receptor-associated factor 6 (TRAF6). This results in the activation of several downstream signaling cascades, including the NFκB, mitogen-activated protein kinases (MAPK), activating protein 1 (AP-1), and nuclear factor of activated T cells (NFATc1), resulting in the formation of multinucleated bone-resorbing osteoclasts. RANKL is widely expressed in skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas.

Immobilized RANKL at 2μg/ml (100 μl/well) can bind Human OPG-His.

The ED50 of RANKL is 0.15 ug/ml .

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight
Formula
CAS Number
Purity >90%, Endotoxin < 0.1 ng/ug (1 EU/ug)
Solubility Reconstituted in ddH2O or PBS at 100 μg/mL
Storage at -20°C
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