Species: Human
Expression system: E.coli
Purity: > 95% as determined by reduced SDS-PAGE
Endotoxin: less than 0.001 ng/ug (0.01 EU/ug) as determined by LAL
Storage and stability: Lyophilized proteins should be stored at < -20°C but can be stored at room temperature for up to 3 weeks. The recombinant protein solution can be stored at 4-7℃ for 2-7 days. The isometric solution of the recombinant sample was stable at < -20°C for 3 months.
Biological activity: Prolactin (PRL) is a neuroendocrine hormone secreted by the pituitary gland. It mainly acts on the mammary gland to promote lactation, but has multiple effects on both men and women. Non-glycosylated prolactin is produced by the pituitary gland and is packaged into storage granules prior to secretion, whereas glycosylated prolactin is reported to be structurally secreted, has a lower biological potency and is removed more quickly from the cycle. Prolactin is mainly synthesized by the anterior pituitary gland in all mammals and its secretion is inhibited by dopamine tonic in the hypothalamus. In humans, prolactin is also peripheral. Prolactin receptor (PRLR) is a transmembrane type I glycoprotein belonging to the cytokine hematopoietic receptor family. The prolactin molecule is thought to bind two receptor molecules. In addition to its lactation activity, peripheral prolactin plays an important role in the development of breast cancer and prostate cancer, reproductive function regulation and immune regulation.
| Solubility (25°C) | It is not recommended to reconstitute to a concentration less than 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[2] Sophie Bernichtein, et al. J Biol Chem. Development of pure prolactin receptor antagonists
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