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Recombinant Human PDGF-AA protein (E.coli)

Cat. No. M25532
Recombinant Human PDGF-AA protein (E.coli) Structure
Synonym:

Platelet-Derived Growth Factor AA

Size Price Availability Quantity
10ug USD 250  USD250 In stock
50ug USD 640  USD640 In stock
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Quality Control & Documentation
  • Purity: >90%, Endotoxin < 1 EU/µg
  • COA
  • MSDS
Biological Activity

Recombinant Human PDGF-AA Protein is a potent mitogen for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. Recombinant Human Platelet-Derived Growth Factor AA (E.coli) expressed the target gene encoding Ser87-Thr211.


Accession: P04085

APMol Mass: 16 KDa, reducing conditions

Endotoxin < 1 EU/µg

Lyophilized from a 0.2 μm filtered solution of 20mM Glycine-HCl, 6% Sucrose, 4% Mannitol, 0.02% Tween 80, pH 3.0.

Chemical Information
Solubility (25°C) Dissolve the lyophilized protein in distilled water. It is not recommended to reconstitute to a concentration less than 100μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at ≤ -20°C for 3 months.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Hongbo Li, et al. Protein Expr Purif. High level expression, efficient purification and bioactivity assay of recombinant human platelet-derived growth factor AA dimer (PDGF-AA) from methylotrophic yeast Pichia pastoris

[2] F Lustig, et al. Biochemistry. Alternative splicing determines the binding of platelet-derived growth factor (PDGF-AA) to glycosaminoglycans

[3] L J Fretto, et al. J Biol Chem. Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor

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Keywords: Recombinant Human PDGF-AA protein (E.coli), Platelet-Derived Growth Factor AA supplier, Recombinant Proteins, inhibitors, activators


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