Activity: Fixed human PD-L1(His tag) at a concentration of 5μg/mL (100 μL/ well) can bind human PD-1(Fc tag) in a linear range of 0.0625-1ug/mL.
Expression host: human HEK293 cells
Molecular weight: Recombinant human PD-1 protein contains 380 amino acids and has a predicted molecular weight of 43.4 kD.
Purity: ≥95% Was analyzed by SDS-PAGE gel and HIGH performance liquid chromatography.
Endotoxin: < 0.1EU/μg.
Stability: Freeze-dried samples can be stored at 4℃ for 24 months, dissolved liquid can be stored at -20℃ for 6-12 months, and avoid repeated freeze-thaw.
Programmed cell death protein 1 (PD-1), also known as CD279 and PDCD1, is a type I membrane protein that is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, bone marrow cells and thymocyte subsets. Pd-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. Pd-l1 is expressed in almost all mouse tumor cell lines, including PA1 myeloma, P815 mast cell tumor, and B16 melanoma, after treatment with IFN-γ. Pd-l2 expression was more restricted and was mainly expressed by DC and a few tumor lines. PD1 inhibits T cell proliferation and related cytokine production, including IL-1, IL-4, IL-10 and IFN-γ, by inhibiting the activation and transduction of PI3K/AKT pathway.
| Solubility (25°C) | ddH2O or PBS |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] Qingqing Xiao, et al. Acta Pharm Sin B. Biological drug and drug delivery-mediated immunotherapy
[5] Y Latchman, et al. Nat Immunol. PD-L2 is a second ligand for PD-1 and inhibits T cell activation
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