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Recombinant Human Osteopontin/SPP1 Protein (Mammalian, C-6His)

Cat. No. M25440
Recombinant Human Osteopontin/SPP1 Protein (Mammalian, C-6His) Structure
Synonym:

OPN; Secreted Phosphoprotein 1

Size Price Availability Quantity
50ug USD 275  USD275 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 1 EU/μg
  • COA
  • MSDS
Biological Activity

Osteopontin/SPP1 is a secreted multifunctional glycoprotein. Its putative functions include roles in bone metabolism, immune regulation, wound healing, cell survival, and tumor progression. Osteopontin/SPP1 acts as a cytokine that is involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. It is essential in the pathway that leads to type I immunity. Osteopontin has been implicated as an important factor in bone remodeling. Specifically, research suggests it plays a role in anchoring osteoclasts to the mineral matrix of bones. The fact that SPP1 interacts with multiple cell surface receptors which are ubiquitously expressed makes it an active player in many physiological and pathological processes including wound healing, bone turnover, tumorigenesis, inflammation and ischemia.

Recombinant Human Osteopontin/SPP1 is produced by Mammalian expression system and the target gene encoding Ile17-Asn314 is expressed with a 6His tag at the C-terminus.


Accession: P10451

APMol Mass: 60-70 KDa, reducing conditions

Endotoxin: < 1 EU/µg as determined by LAL test

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Chemical Information
Solubility (25°C) Dissolve the lyophilized protein in distilled water. It is not recommended to reconstitute to a concentration less than 100μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Itai Rotem, et al. Basic Res Cardiol. Osteopontin promotes infarct repair

[2] Qipeng Wu, et al. Cell Death Dis. Osteopontin promotes hepatocellular carcinoma progression through inducing JAK2/STAT3/NOX1-mediated ROS production

[3] Rulan Jiang, et al. Mol Nutr Food Res. Recombinant Bovine and Human Osteopontin Generated by Chlamydomonas reinhardtii Exhibit Bioactivities Similar to Bovine Milk Osteopontin When Assessed in Mouse Pups Fed Osteopontin-Deficient Milk

[4] R Zohar, et al. Matrix Biol. Flow cytometric analysis of recombinant human osteogenic protein-1 (BMP-7) responsive subpopulations from fetal rat calvaria based on intracellular osteopontin content

[5] J W Xuan, et al. J Cell Biochem. Recombinant GST-human osteopontin fusion protein is functional in RGD-dependent cell adhesion

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Keywords: Recombinant Human Osteopontin/SPP1 Protein (Mammalian, C-6His), OPN; Secreted Phosphoprotein 1 supplier, Recombinant Proteins, inhibitors, activators


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