Recombinant Human Noggin Protein (HEK293) can bind to ligands of the transforming growth factor-β family and regulate its activity by inhibiting its pathway with signal receptors. Noggin was initially identified as a BMP-4 antagonist, and its effect is essential for the normal formation of the head and other back structures. Therefore, noggin has been shown to regulate the activities of other BMP-2, -7, -13 and -14. Transgenic mice overexpress noggin in mature osteoblasts, which is manifested by impaired differentiation of osteoblasts, decreased bone formation, and severe osteoporosis.
Determined by the ability of ATDC chondrocytes to inhibit the production of alkaline phosphatase by 5.0 ng/ml BMP-4, the expected ED50 of this effect is about 2.0-3.0 ng/ml.
The DNA sequence encoding human noggin (NP_005441.1) is expressed with a His tag at the C terminus.
Recombinant human noggin contains 211 amino acids and has a predicted theoretical molecular weight of 23.9 kd.
Endotoxin ≤0.01EU/ug
Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. |
Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
Related Recombinant Proteins Products |
---|
Recombinant Human IL-10 (CHO)
Interleukin-10 (IL-10) is usually secreted as a homodimer and, upon binding to its receptor, inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and Th2 cells. |
Recombinant Human IL-5 (CHO)
Interleukin-5 (IL-5) is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF). IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. |
Recombinant Human Tau-F Protein (E.coli)
Tau proteins are proteins which contain four Tau/MAP repeats. They promote microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. |
Recombinant Human Tau Protein (E. coli, His Tag)
Tau proteins are proteins that stabilize microtubules. Tau protein is a highly soluble microtubule-associated protein (MAP). In humans, these proteins are mostly found in neurons compared to non-neuronal cells. One of tau's main functions is to modulate the stability of axonal microtubules. |
Recombinant Rat IL-23 Protein (HEK293, C-His)
IL-23 (IL-23 alpha & IL-12 beta Heterodimer) Protein is a recombinant protein dimer complex containing rat-derived IL-23 alpha & IL-12 beta Heterodimer protein, expressed by HEK293. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.