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Recombinant Human Neurolysin Protein (E. coli, His Tag)

Cat. No. M45322

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Human Neurolysin Protein (E. coli, His Tag) Structure
Synonym:

NLN

Size Price Availability Quantity
50ug USD 720  USD720 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 1 EU/µg
  • COA
  • MSDS
Biological Activity

The recombinant human Neurolysin/NLN consists of 685 amino acids and predicts a molecular mass of 78.7 kDa. A DNA sequence encoding the human NLN (Ser38-Pro704) is expressed with a polyhistidine tag at the N-terminus.

Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl)acetyl Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH, the specific activity is > 100 pmol/min/μg.


Endotoxin < 1 EU/µg

Apparent Molecular Weight: 67 kDa, reducing conditions

Lyophilized from sterile PBS, pH 7.4.

Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
References

[1] Srinidhi Jayaraman, et al. J Pharmacol Exp Ther. Identification and Characterization of Two Structurally Related Dipeptides that Enhance Catalytic Efficiency of Neurolysin

[2] Vardan T Karamyan. Med Hypotheses. Peptidase neurolysin is an endogenous cerebroprotective mechanism in acute neurodegenerative disorders

[3] M Ursula Norman, et al. Am J Physiol Heart Circ Physiol. Endopeptidases 3.4.24.15 and 24.16 in endothelial cells: potential role in vasoactive peptide metabolism

[4] V Oliveira, et al. Biochemistry. Substrate specificity characterization of recombinant metallo oligo-peptidases thimet oligopeptidase and neurolysin

[5] V Rioli, et al. Biochem Biophys Res Commun. Neuropeptide specificity and inhibition of recombinant isoforms of the endopeptidase 3.4.24.16 family: comparison with the related recombinant endopeptidase 3.4.24.15

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Keywords: Recombinant Human Neurolysin Protein (E. coli, His Tag), NLN supplier, Cytokines and Growth Factors, inhibitors, activators

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