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Recombinant Human NANS (E.coli, N-6His)

Cat. No. M21617

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Recombinant Human NANS (E.coli, N-6His) Structure
Synonym:

N-Acetylneuraminate Synthase

Size Price Availability Quantity
10ug USD 185  USD185 In stock
50ug USD 455  USD455 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 1 EU/μg
  • COA
  • MSDS
Biological Activity

N-Acetylneuraminate Synthase (NANS) is an enzyme that contains one AFP-like domain. NANS is ubiquitous and plays a role in the biosynthetic pathways of sialic acids. Recombinant Human N-Acetylneuraminate Synthase is produced by E.coli expression system and the target gene encoding Met1-Ser359 is expressed with a 6His tag at the N-terminus. NANS produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). It also can use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.

Chemical Information
Solubility (25°C) Water
Saline
PBS
Storage Store at ≤-70°C, stable for 6 months after receipt
References

[1] S Feuerbaum, et al. Int J Med Microbiol. De-O-Acetylation of mucin-derived sialic acids by recombinant NanS-p esterases of Escherichia coli O157:H7 strain EDL933

[2] Nadja Saile, et al. Int J Med Microbiol. Growth advantage of Escherichia coli O104:H4 strains on 5-N-acetyl-9-O-acetyl neuraminic acid as a carbon source is dependent on heterogeneous phage-Borne nanS-p esterases

[3] Nadja Saile, et al. Appl Environ Microbiol. Escherichia coli O157:H7 Strain EDL933 Harbors Multiple Functional Prophage-Associated Genes Necessary for the Utilization of 5-N-Acetyl-9-O-Acetyl Neuraminic Acid as a Growth Substrate

[4] Chatchawal Phansopa, et al. Biochem J. Characterization of a sialate-O-acetylesterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid release by NanH, its cognate sialidase

[5] Michael John Aldape, et al. J Infect Dis. The leukemoid reaction in Clostridium sordellii infection: neuraminidase induction of promyelocytic cell proliferation

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Keywords: Recombinant Human NANS (E.coli, N-6His), N-Acetylneuraminate Synthase supplier, Cytokines and Growth Factors, inhibitors, activators

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