Species: Human
Expression system: E. coli
Purity: SDS-PAGE analysis > 97%, HPLC analysis > 97%
Endotoxin: protein content < 1 EU/μg by LAL method
Theoretical molecular weight: 12.4 kDa
Storage and stability: After receiving, the product remains stable at -70°C or -20°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. Avoid repeated freeze-thaw cycles.
Bioactivity: Fully bioactive compared to the standard. The bioactivity of chemotactic bioassays using human lymphocytes was in the concentration range of 1.0-10.0 ng/ mL. Human CCL28 cDNA encodes a 127 amino acid (AA) residue precursor protein and a putative 22 AA residue signal peptide cleaved to yield 105 AA residue mature proteins. Human CCL28 and mouse CCL28 are highly conserved, sharing 83% aa homology in mature regions. Among CC chemokines, CCL28 had the highest homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression was highest in normal and pathological colons, and the protein was expressed by epithelial cells. Human CL28 RNA is also present in normal and asthmatic lung tissue. The receptor for CCL28 has been identified as CCR10 (GPR2 orphan receptor), which is also the receptor for CCL27/CTACK.
| Solubility (25°C) | Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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