Species: Human
Expression system: CHO
Purity: sdS-PAGE analysis result was 98%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: 10~18 kDa, sdS-PAGE under reduction conditions
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: The EC50 value of human McP-3 /CCL7 against Cho-K1 / Gα15/hCCR1 cells (human Gα15 and human CCR1 expressed stably in Cho-K1 cells) was less than 1.5 μ G/mL. Because CCL7 has two adjacent N-terminal cysteine residues in its mature form, it is classified in a subfamily of chemokines known as CC chemokines. CCL7 specifically attracts monocytes and regulates macrophage function. It is produced by certain tumor cell lines and macrophages. This chemokine is located on human chromosome 17 and is most closely related to CCL2 in a large cluster containing many other CC chemokines. CCL7 signals through CCR1, CCR2, and CCR3 receptors.
| Solubility (25°C) | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[2] C Blanpain, et al. Blood. CCR5 binds multiple CC-chemokines: MCP-3 acts as a natural antagonist
| Related Recombinant Proteins Products |
|---|
| Recombinant Human Serpin A12 (E.coli, N-GST)
Vaspin (Visceral Adipose-Specific SERPIN) is a newly described adipokine. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. |
| Recombinant Human B2M Protein (Mammalian, C-6His)
β-2-Microglobulin (B2M) is a secreted protein with 1 Ig-like C1-type (immunoglobulin-like) domain which belongs to the beta-2-microglobulin family. B2M may adopt the fibrillar configuration of amyloid in certain pathologic states. |
| Recombinant Mouse E-Selectin/CD62E Protein (HEK293)
E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. |
| Recombinant Human DPP4/CD26 (Mammalian, C-6His)
DPP4/CD26 is a signal-anchor for type II membrane protein that belongs to the peptidase S9B family. DPP4/CD26 acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. It’s binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. |
| Recombinant Human USP14 (E.coli, N-6His)
USP14 belongs to the ubiquitin-specific processing (USP) family which is a deubiquitinating enzyme (DUB) with His and Cys domains. USP14 acts also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.
