Recombinant Human LIF Protein promotes the long-term maintenance of embryonic stem cells by inhibiting the spontaneous differentiation of embryonic stem cells. LIF is a pleiotrophic factor produced by a variety of cell types, including T cells, myeloid cell lines, fibroblasts, liver, heart, and melanoma. Human LIF is active on mouse cells and is widely used to maintain the spontaneous differentiation of mouse ESCs, while mouse LIF cannot function on human cells because it cannot bind to human LIF receptors.
The recombinant human LIF protein contains 186 amino acids and the predicted molecular weight is 20.5kd.
Depends on its ability to stimulate the proliferation of human TF-1 cells, expected ED50 is ≤0.1 ng/ml, corresponding to activity ≥1×107 IU/mg.
Expression host is Human HEK293 cells
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Purity||≥95%, Endotoxin ≤0.01EU/ug|
|Storage||2-8°C, dry, sealed|
|Related Cytokines Products|
|Recombinant Human TGF-α (E. coli)
Recombinant Human TGF-α, also known as sarcoma growth factor, TGF-type I and ETGF, is a member of the EGF family of cytokines. TGF-α signals through EGFR and acts synergistically with TGF-beta to promote the proliferation of a wide range of epidermal and epithelial cells.
|Recombinant Human EGF (E. coli)
Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells.
|Recombinant Human FGF-10/KGF-2 (E. coli)
Recombinant Human Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in the lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares homology with KGF and receptor binding to FGFR2-IIIb.
|Recombinant Human HGF (CHO-expressed)
Recombinant human Hepatocyte Growth Factor (rhHGF) , also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily). It is produced by mesenchymal cells and acts on epithelial cells, endothelial cells and haemopoietic progenitor cells.
|Recombinant Human Glial Cell Line-derived Neurotrophic Factor
Glial cell-derived neurotrophic factor is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.