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Recombinant Human LIF Protein

Cat. No. M9998
Recombinant Human LIF Protein Structure
Synonym:

Leukemia Inhibitory Factor

Size Price Availability Quantity
50ug USD 324  USD360 In stock
100ug USD 504  USD560 In stock
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Quality Control
  • Current batch:
  • Purity ≥95%, Endotoxin ≤0.01EU/ug
  • COA
  • MSDS
Biological Activity

Recombinant Human LIF Protein promotes the long-term maintenance of embryonic stem cells by inhibiting the spontaneous differentiation of embryonic stem cells. LIF is a pleiotrophic factor produced by a variety of cell types, including T cells, myeloid cell lines, fibroblasts, liver, heart, and melanoma. Human LIF is active on mouse cells and is widely used to maintain the spontaneous differentiation of mouse ESCs, while mouse LIF cannot function on human cells because it cannot bind to human LIF receptors. 

The recombinant human LIF protein contains 186 amino acids and the predicted molecular weight is 20.5kd. 

Depends on its ability to stimulate the proliferation of human TF-1 cells, expected ED50 is ≤0.1 ng/ml, corresponding to activity ≥1×107 IU/mg. 

Expression host is Human HEK293 cells

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight
Formula
CAS Number
Purity ≥95%, Endotoxin ≤0.01EU/ug
Solubility Water
Saline
PBS
Storage 2-8°C, dry, sealed
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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Recombinant Human LIF Protein, Leukemia Inhibitory Factor supplier, Cytokines, inhibitors

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