Recombinant Human LIF Protein (HEK293) promotes the long-term maintenance of embryonic stem cells by inhibiting the spontaneous differentiation of embryonic stem cells. LIF is a pleiotrophic factor produced by a variety of cell types, including T cells, myeloid cell lines, fibroblasts, liver, heart, and melanoma. Human LIF is active on mouse cells and is widely used to maintain the spontaneous differentiation of mouse ESCs, while mouse LIF cannot function on human cells because it cannot bind to human LIF receptors.
The recombinant human LIF protein contains 186 amino acids and the predicted molecular weight is 20.5kd.
Depends on its ability to stimulate the proliferation of human TF-1 cells, expected ED50 is ≤0.1 ng/ml, corresponding to activity ≥1×107 IU/mg.
Endotoxin ≤0.01EU/ug
Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS to 100 μg/ml. |
Storage | Stored at ≤-20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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