Free shipping on all orders over $ 500

Recombinant Human LIF Protein (E.coli)

Cat. No. M11370

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Human LIF Protein (E.coli) Structure
Synonym:

Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor

Size Price Availability Quantity
50ug USD 465  USD465 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
  • Purity: >95%, Endotoxin <0.01 EU/μg
  • COA
  • MSDS
Biological Activity

Leukemia suppressor factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by inhibiting spontaneous differentiation. LIF also has a variety of activities such as cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitosis of some factor dependent cell lines, and promotion of megakaryocyte production in vivo. Human and mouse mature LIFs have 78% sequence homology at the amino acid level. Human LIF is also active in human and mouse cells. Mouse LIF is about 1000 times more active against human cells than human LIF.

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells, the ED50 for this effect is 25-150 pg/ml.

Recombinant Human Leukemia Inhibitory Factor expressed the target gene encoding Ser23-Phe202.


Accession: P15018

Endotoxin < 0.01 EU/µg

Apparent Molecular Weight: 18 KDa, reducing conditions

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
References

[1] Hee Jung Son, et al. Spine J. Efficacy and safety of Escherichia coli-derived recombinant human bone morphogenetic protein-2 in additional lumbar posterolateral fusion: minimum 1-year follow-up

[2] Amelie Croset, et al. J Biotechnol. Differences in the glycosylation of recombinant proteins expressed in HEK and CHO cells

[3] Christa Broholm, et al. J Appl Physiol (1985). LIF is a contraction-induced myokine stimulating human myocyte proliferation

[4] A Jurisicova, et al. Fertil Steril. Recombinant human leukemia inhibitory factor does not enhance in vitro human blastocyst formation

[5] D P Gearing, et al. Ciba Found Symp. Reconstitution of high affinity leukaemia inhibitory factor (LIF) receptors in haemopoietic cells transfected with the cloned human LIF receptor

Related Cytokines and Growth Factors Products
Recombinant Human GDF-15 Protein (HEK293 N-hFc)

Growth-differentiation factor 15 (GDF15), also known as MIC-1, is a secreted member of the transforming growth factor (TGF)-β superfamily. GDF-15 has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF-15 overexpression arising from an expanded erythroid compartment contributes to iron overload in thalassemia syndromes by inhibiting hepcidin expression.

Recombinant Human FGFR1 Protein (HEK293, C-His)

FGFR1, also known as CD331, is a full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain.

Recombinant Human FGFR2 Protein (HEK293, C-His)

FGFR2, also known as CD332, acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts.

Recombinant Mouse BMP-4 Protein (E. coli, C-His)

Bone Morphogenetic Protein-4 (BMP-4) is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP-4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog to establish a dorsal-ventral axis for the differentiation of later structures.

Recombinant Human Coagulation Factor X (HEK293, C-Fc)

Coagulation factor X, belongs to the peptidase S1 family. Coagulation factor X is initially synthesized in the liver. Coagulation factor X is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

  Catalog
Abmole Inhibitor Catalog




Keywords: Recombinant Human LIF Protein (E.coli), Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor supplier, Cytokines and Growth Factors, inhibitors, activators

All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.



Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.