Leukemia suppressor factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by inhibiting spontaneous differentiation. LIF also has a variety of activities such as cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitosis of some factor dependent cell lines, and promotion of megakaryocyte production in vivo. Human and mouse mature LIFs have 78% sequence homology at the amino acid level. Human LIF is also active in human and mouse cells. Mouse LIF is about 1000 times more active against human cells than human LIF.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells, the ED50 for this effect is 25-150 pg/ml.
Recombinant Human Leukemia Inhibitory Factor expressed the target gene encoding Ser23-Phe202.
Accession: P15018
Endotoxin < 0.01 EU/µg
Apparent Molecular Weight: 18 KDa, reducing conditions
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
| Form | Lyophilized powder |
| Solubility (25°C) | Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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