Free shipping on all orders over $ 500

Recombinant Human LIF Protein (E.coli)

Cat. No. M11370

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Human LIF Protein (E.coli) Structure
Synonym:

Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor

Size Price Availability Quantity
50ug USD 465  USD465 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
  • Purity: >95%, Endotoxin <0.01 EU/μg
  • COA
  • MSDS
Biological Activity

Leukemia suppressor factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by inhibiting spontaneous differentiation. LIF also has a variety of activities such as cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitosis of some factor dependent cell lines, and promotion of megakaryocyte production in vivo. Human and mouse mature LIFs have 78% sequence homology at the amino acid level. Human LIF is also active in human and mouse cells. Mouse LIF is about 1000 times more active against human cells than human LIF.

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells, the ED50 for this effect is 25-150 pg/ml.

Recombinant Human Leukemia Inhibitory Factor expressed the target gene encoding Ser23-Phe202.


Accession: P15018

Endotoxin < 0.01 EU/µg

Apparent Molecular Weight: 18 KDa, reducing conditions

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
References

[1] Hee Jung Son, et al. Spine J. Efficacy and safety of Escherichia coli-derived recombinant human bone morphogenetic protein-2 in additional lumbar posterolateral fusion: minimum 1-year follow-up

[2] Amelie Croset, et al. J Biotechnol. Differences in the glycosylation of recombinant proteins expressed in HEK and CHO cells

[3] Christa Broholm, et al. J Appl Physiol (1985). LIF is a contraction-induced myokine stimulating human myocyte proliferation

[4] A Jurisicova, et al. Fertil Steril. Recombinant human leukemia inhibitory factor does not enhance in vitro human blastocyst formation

[5] D P Gearing, et al. Ciba Found Symp. Reconstitution of high affinity leukaemia inhibitory factor (LIF) receptors in haemopoietic cells transfected with the cloned human LIF receptor

Related Cytokines and Growth Factors Products
Recombinant Human FGF-8b Protein (E. coli)

Recombinant Human FGF-8b Protein is a heparin-binding growth factor, which plays a core role in prenatal development, postnatal growth and regeneration of various tissues by promoting cell proliferation and other effect.

Recombinant Rat GDNF Protein (Baculovirus-Insect, N-His)

GDNF is an important member of the GDNF family of ligands. GDNF plays a key role in the promotion of the survival of dopaminergic neurons. GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein also promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption.

Recombinant Rat PDGF-BB (E. coli)

Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic.

Recombinant Human ApoE3 Protein (HEK293, C-6His)

ApoE is a constituent of a subclass of high density of lipoproteins (HDL) involved in cholesterol transport. ApoE mediates high affinity binding of chylomicrons and vLDL particles to the LDL receptor, allowing for specific uptake of these particles by the liver, preventing the accumulation of cholesterol rich particles in the plasma.

Recombinant Mouse Granzyme B (HEK293, C-6His)

Granzyme B (GZMB) is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp and seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. The protein cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

  Catalog
Abmole Inhibitor Catalog




Keywords: Recombinant Human LIF Protein (E.coli), Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor supplier, Cytokines and Growth Factors, inhibitors, activators

All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.



Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.