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Recombinant Human KRAS (G12D, E. coli, His)

Cat. No. M11234
Recombinant Human KRAS (G12D, E. coli, His) Structure
Synonym:

Recombinant Human KRAS G12D (E. coli, His)

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50ug USD 440  USD440 In stock
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Quality Control & Documentation
  • Purity: >90%, Endotoxin < 1 EU/μg
  • COA
  • MSDS
Biological Activity

The KRAS gene provides instructions for making a protein called K-Ras, part of the RAS/MAPK pathway. The K-Ras protein is a GTPase, which means it converts a molecule called GTP into another molecule called GDP. In this way the K-Ras protein acts like a switch that is turned on and off by the GTP and GDP molecules. 


Accession #: P01116-2 

Molecular Weight: 23.8 kDa 

Endotoxin: ≤ 1 EU/μg 

Lyophilized from a 0.2 μm filtered solution in PBS.

Chemical Information
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml.
Storage Stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Yongde Luo, et al. Gastroenterology. Oncogenic KRAS Reduces Expression of FGF21 in Acinar Cells to Promote Pancreatic Tumorigenesis in Mice on a High-Fat Diet

[2] Callum P Rakhit, et al. Dis Model Mech. Early detection of pre-malignant lesions in a KRAS G12D-driven mouse lung cancer model by monitoring circulating free DNA

[3] Gal Cafri, et al. Nat Commun. Memory T cells targeting oncogenic mutations detected in peripheral blood of epithelial cancer patients

[4] Shanmin Zhao, et al. Cell Physiol Biochem. KRAS (G12D) cooperates with AML1/ETO to initiate a mouse model mimicking human acute myeloid leukemia

[5] Victoria A Appleman, et al. Mol Cancer Res. KRAS(G12D)- and BRAF(V600E)-induced transformation of murine pancreatic epithelial cells requires MEK/ERK-stimulated IGF1R signaling

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