Expression system: E.coli
Purity: >95% as determined by SDS-PAGE
Protein Construct: DNA sequence encoding human IL32 (Ala31-Lys234) fused with His tag
Predicted molecular weight: 25.56 kDa
Storage and Stability: Use a manually thawed refrigerator to avoid repeated freeze-thaw cycles. Store at 2 to 8 °C for one week. Store at -20 to -80 °C for 12 months from date of receipt.
Bioactivity: It induces various cytokines such as TNFA/TNF-α and IL8. it activates the typical cytokine signaling pathways NF-kappa-B and p38 MAPK.
|Solubility (25°C)||Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products
please refer to it for details.
Powder -20°C 3 years ; 4°C 2 years
In solvent -80°C 6 months ; -20°C 1 month
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
 Zhao-ju Tian, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. [Cloning and expression of human interleukin-32 and studies on its bioactivity]
 Siyoung Lee, et al. Hybridoma (Larchmt). Interleukin-32 gamma specific monoclonal antibody and developing IL-32 specific ELISA
 Jun Jaekal, et al. Vet Immunol Immunopathol. Cloning and characterization of bovine interleukin-32 beta isoform
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