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Genus: Human
Expression system: E.coli
Purity: >95% as determined by SDS-PAGE
Protein Construct: DNA sequence encoding human IL32 (Ala31-Lys234) fused with His tag
Predicted molecular weight: 25.56 kDa
Storage and Stability: Use a manually thawed refrigerator to avoid repeated freeze-thaw cycles. Store at 2 to 8 °C for one week. Store at -20 to -80 °C for 12 months from date of receipt.
Bioactivity: It induces various cytokines such as TNFA/TNF-α and IL8. it activates the typical cytokine signaling pathways NF-kappa-B and p38 MAPK.
| Solubility (25°C) | Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products please refer to it for details. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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