Protoheparin binds EGF like growth factor (HB-EGF), also known as DTR, DTS and HEGFL, and is a member of the EGF family mitogen. It is expressed in macrophages, monocytes, endothelial cells and muscle cells. HB-EGF sends signals through EGF receptor to stimulate the proliferation of smooth muscle cells, epithelial cells and keratinocytes. Compared with EGF, HB-EGF binds to EGF receptor with higher affinity and is proved to be more mitogenic, which may be due to its ability to bind to heparin and heparin sulfate proteoglycan. HB-EGF has also been reported as diphtheria toxin receptor, which mediates the endocytosis of conjugated toxins.
ED50 < 0.75 ng/ml, measured in a cell proliferation assay using 3T3 cells.
Accession: # Q99075。
Apparent molecular weight: 12~14kDa, SDS-PAGE analysis under reducing conditions
Lyophilized after extensive dialysis against PBS.
| Solubility (25°C) | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS to 100 μg/ml. |
| Storage | Stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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