Recombinant Human Fibronectin is an extracellular glycoprotein. As one of the major cell adhesion molecules, fibronectin plays a key role in many important physiological processes, such as embryogenesis, wound healing, hemostasis and thrombosis. Fibronectin can promote cell growth, increase cell apposition rate, enhance cell metabolism level and shorten cell growth time. Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.
Molecular Weight: 200~210 kDa
Endotoxin: < 0.5 EU/μg
Supplied as lyophilized powder, suitable for cell culture
|Solubility (25°C)||Reconstitute the lyophilized powder in PBS (pH 7.2~7.4) to prepare a 1 mg/mL stock solution. Divide the stock solution into individual dosages and stored at -20 ℃.|
|Storage||Stored at -20°C for up to 2 years. The stock solution should be stable for up to 3 months at -20°C.|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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