Species: Human
Expression system: E.coli
Purity: > 95% as determined by reduced SDS-PAGE
Endotoxin: less than 0.001 ng/ug (0.01 EU/ug) as determined by LAL
Storage and stability: Lyophilized proteins should be stored at < -20°C but can be stored at room temperature for up to 3 weeks. The recombinant protein solution can be stored at 4-7℃ for 2-7 days. The isometric solution of the recombinant sample was stable at < -20°C for 3 months.
Bioactivity: Fibroblast growth factor 4(FGF-4) is a heparin-binding member of the FGF family. Human FGF4 cDNA encodes 206 amino acids (AA), including 33 signal sequences and 173 mature proteins, with a FGF homologous domain containing a heparin binding region near the C-terminal. The homology of mature human FGF4 with mouse, rat, dog and bovine FGF4 was 91%, 82%, 94% and 91%, respectively. Human FGF-4 has been shown to have cross-species activity. The expression of FGF-4 and its receptors, FGF R1c, 2C, 3C and 4, are spatio-temporal regulated during embryonic development. Fgf-4 is thought to play a physiologically relevant role in the self-renewal of human embryonic stem cells. It promotes stem cell proliferation, but may also aid differentiation depending on environment and concentration, and is often included in embryonic stem cell culture medium in vitro. In vitro, FGF-4 is a mitotic source of fibroblasts and endothelial cells and has the potential of autocrine transformation.
| Solubility (25°C) | It is not recommended to reconstitute to a concentration less than 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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