All AbMole products are for research use only, cannot be used for human consumption.
Species: Human
Expression system: E. coli
Purity: SDS-PAGE analysis, > 95%, HIGH performance liquid chromatography analysis, > 95%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: ~19.5 kDa, sdS-PAGE under reduction conditions
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: ED50 < 0.5µg/ mL, niH-3T3 cells were used for cell proliferation assay in the presence of mouse Klotho 1.25µg/ mL and heparin 10µg/ mL, corresponding specific activity > 2.0 × 103 units /mg. Along with FGF-19/15 and FGF-23, FGF-21 is classified as a member of the atypical FGF-subfamily because it must bind to the Klotho coreceptor in order to bind to the FGF-receptor and activate downstream signaling pathways. In vivo, FGF-21 is expressed in liver, pancreas, adipose tissue and skeletal muscle and plays a central role in energy metabolism. Fgf-21 expression is stimulated by free fatty acids and insulin resistance status, and is associated with systemic insulin resistance. Fgf-21 activates glucose uptake by fat cells and increases insulin sensitivity, suggesting that it is a new target with potential anti-diabetic properties.
| Solubility (25°C) | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
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All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
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