Species: Human
Expression system: E. coli
Purity: SDS-PAGE analysis, > 95%, HIGH performance liquid chromatography analysis, > 95%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: ~19.5 kDa, sdS-PAGE under reduction conditions
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: ED50 < 0.5µg/ mL, niH-3T3 cells were used for cell proliferation assay in the presence of mouse Klotho 1.25µg/ mL and heparin 10µg/ mL, corresponding specific activity > 2.0 × 103 units /mg. Along with FGF-19/15 and FGF-23, FGF-21 is classified as a member of the atypical FGF-subfamily because it must bind to the Klotho coreceptor in order to bind to the FGF-receptor and activate downstream signaling pathways. In vivo, FGF-21 is expressed in liver, pancreas, adipose tissue and skeletal muscle and plays a central role in energy metabolism. Fgf-21 expression is stimulated by free fatty acids and insulin resistance status, and is associated with systemic insulin resistance. Fgf-21 activates glucose uptake by fat cells and increases insulin sensitivity, suggesting that it is a new target with potential anti-diabetic properties.
Solubility (25°C) | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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