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Recombinant Human EpCAM/Trop1 (Expi293, His)

Cat. No. M15093
Recombinant Human EpCAM/Trop1 (Expi293, His) Structure

CD326 antigen; CD326; EGP; EGP-2

Size Price Availability Quantity
100ug USD 500  USD500 In stock
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Quality Control & Documentation
  • Purity: >95% , Endotoxin ≤ 1 EU/μg
  • COA
  • MSDS
Biological Activity

Species: Human
Expression system: Expi293
Purity: SDS-PAGE analysis > 95%, HPLC analysis > 95%
Endotoxin: LAL method for determination of protein ≤1 EU/μg
Apparent molecular weight: 30 ~ 45 kDa, reduced under SDS-PAGE
Storage and stability: After receiving, the product remains stable at -70°C or -20°C for up to 6 months. Avoid repeated freeze-thaw cycles

Bioactivity: 0.2 μg/ mL (100 μ L /Well) immobilized human EpCAM. The dose-response curve of anti-Trop1 antibody EC50 was 8.7 ng/ mL by ELISA. Epithelial cell adhesion molecule (EpCAM), also known as KS1/4, GP40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein, It consists of an extracellular domain of 242 amino acids (AA) (with two EGF-like repeats), a 23 AA transmembrane segment, and a 26 AA cytoplasmic domain.

Chemical Information
Solubility (25°C) It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

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Keywords: Recombinant Human EpCAM/Trop1 (Expi293, His), CD326 antigen; CD326; EGP; EGP-2 supplier, Recombinant Proteins, inhibitors, activators

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