Expression system: E. coli
Purity: SDS-PAGE analysis > 97%, HPLC analysis > 97%
Endotoxin: protein content < 1 EU/μg by LAL method
Theoretical molecular weight: 8.8 kDa
Storage and stability: After receiving, the product remains stable at -70°C or -20°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. Avoid repeated freeze-thaw cycles
Bioactivity: Fully bioactive compared to the standard. The bioactive concentration was 50.0-100.0 ng/ mL by chemotactic bioassay of human peripheral blood eosinophils. Eotaxin-2 selectively chemically attracts cCR3-expressing cells, including eosinophils, basophils, Th2 T cells, mast cells, and certain subpopulations of dendritic cells. In addition, EOtaxin-2 inhibits the proliferation of pluripotent hematopoietic progenitors. The mature protein, which also includes a C-terminal truncation, contains 78 amino acid residues (mouse homolog 92 A.A. Residues, no C-terminal truncation).
| Solubility (25°C) | Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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