Species: Human/Cynomolgus
Expression system: Mammalian
Molecular weight: 16.7 KDa
Purity: > 95% as determined by reduced SDS-PAGE
Endotoxin: LAL test less than 0.1 ng/µg (1 EU/µg)
Storage and stability: The freeze-dried protein should be stored at ≤-20℃ and stored stably for one year after receiving. The recombinant protein solution can be stored at 2-8℃ for 2-7 days. The recombinant samples were stable for 3 months at ≤-20°C.
Bioactivity: t cell-specific surface glycoprotein CD28(CD28) is a single-channel membrane protein containing an IGg-like EV-like (immunoglobulin-like) domain. It belongs to the immunoglobulin (Ig) superfamily. CD28 is a molecule expressed on T cells that provides a costimulatory signal needed for T cell activation. CD28 co-stimulation is necessary for CD4-positive t cell proliferation and survival, interleukin-2 production, and T-helper type 2 development. Human thymus retroregulatory T cells require CD28 co-stimulation in order to expand and maintain effective inhibitory function in vivo. Apoptosis plays a key role in age-related decline in CD28 expression and immunosenescence. CD28 is a receptor for CD80 (B7.1) and CD86 (B7.2). CD80 is upregulated in antigen-presenting cells (APCs) when activated by toll-like receptor ligands. The expression of CD86 on antigen presenting cells is constitutive. CD28 is the only B7 receptor that is constitutively expressed on naive T cells.
| Solubility (25°C) | It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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