Expression system: HEK 293
Purity: SDS-PAGE, > 95%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: 22~ 28kda, sds-page under reduction conditions
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: ED50 < 0.2 μg/ mL and tF-1 cells were used for cell proliferation assay. Subsequent studies have shown that CNTF is a survival factor for other nerve cell types including dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, greater pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent degeneration of motor axons after axonotomy. The human CNTF gene is located in the proximal region of the long arm of chromosome 11. CNTF is highly conserved across species and shows cross-species activity. The homology of human and rat CNTF protein sequences was about 83%. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All four helical bundle cytokines share GP130 as a signal transduction subunit in their receptor complex.
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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