CD40 Ligand (CD40L/CD154/TRAP) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand is a differentiation antigen that is expressed on the surface of T-cells. CD40L has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. Recombinant Human CD40 Ligand is produced by E.coli expression system and the target gene encoding Met113-Leu261 is expressed.
Determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells, the ED50 is 5.0-10.0 ng/ml, corresponding to a specific activity of 1.0 x 105 IU/mg.
Accession: P29965
Apparent Molecular Weight: 15 KDa, reducing conditions
Endotoxin < 1 EU/µg
Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 200mM NaCl, 0.1mM EDTA, pH 8.0.
| Form | Lyophilized powder |
| Solubility (25°C) | Dissolve the lyophilized protein in distilled water. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at ≤ -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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