Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. Human C5a has four α-helices plus three intrachain disulfide bonds that create a triple loop structure. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. Recombinant Human C5a expressed the target gene encoding Thr678-Arg751.
Accession: P01031
Apparent Molecular Weight: 12 KDa, reducing conditions
Endotoxin < 1 EU/µg
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
| Form | Lyophilized powder |
| Solubility (25°C) | Dissolve the lyophilized protein in distilled water. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at ≤ -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[3] Hong Xiao et al. J Am Soc Nephrol. C5a receptor (CD88) blockade protects against MPO-ANCA GN
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