Recombinant Human BMP-2 Protein (HEK293) is a powerful osteoinductive cytokine that can be combined with collagen and synthetic hydroxyapatite and other osteoconductive carriers to induce the formation of bone and cartilage. BMP-2 also plays an important role in cardiac morphogenesis, and is expressed in lung, liver, spleen, prostate, ovary and small intestine and other tissues. The functional form of BMP-2 is a 26 kDa protein, which is composed of two identical 114 amino acid polypeptide chains (monomers) connected by a single disulfide bond. Each bmp-2 monomer is expressed as the C-terminal part of the precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion and a 259 amino acid precursor. After the precursor is dimerized, the covalent bond between the propeptide (which is also a homodimer of disulfide bonds) and the mature BMP-2 ligand is cleaved by the furan-type protease.
The recombinant human BMP-2 protein contains 352 amino acids and the predicted molecular weight is 39.8kd.
Measured by its ability to induce ATDC-5 cells to produce alkaline phosphatase, the ED50 is ≤1μg/mL.
Endotoxin <0.1EU/μg
Expression host is Human HEK293 cells
| Form | Lyophilized powder |
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS to 100 μg/ml. |
| Storage | Stored at ≤-20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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