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Recombinant Human Beta-NGF Protein (E. coli)

Cat. No. M31178

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Human Beta-NGF Protein (E. coli) Structure
Synonym:

β-NGF; NGFB; Beta-Nerve Growth Factor

Size Price Availability Quantity
10ug USD 116  USD116 In stock
50ug USD 310  USD310 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 0.01 EU/μg
  • COA
  • MSDS
Biological Activity

Human β-Nerve Growth Factor (β-NGF) is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity. 

Recombinant Human Beta-NGF is produced by E.coli expression system and expressed the target gene encoding Ser122-Ala241.

Measured by the cell proliferation assay using TF‑1 human erythroleukemic cells, the ED50 is 0.03-0.3 ng/ml.


Accession: P01138

APMol Mass: 14 KDa, reducing conditions

Endotoxin < 0.01 EU/µg

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Chemical Information
Form Lyophilized powder
Solubility (25°C) Dissolve the lyophilized protein in distilled water. It is not recommended to reconstitute to a concentration less than 100μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at ≤ -20°C for 3 months.
References

[1] Zahra Hajihassan, et al. Pol J Microbiol. Improved Production of Recombinant Human β-NGF in Escherichia coli - a Bioreactor Scale Study

[2] Pouria Gholami Tilko, et al. Prep Biochem Biotechnol. Optimization of recombinant β-NGF expression in Escherichia coli using response surface methodology

[3] Wen-Juan Luo, et al. Int J Ophthalmol. Human β-NGF gene transferred to cat corneal endothelial cells

[4] Jingchuan Li, et al. Sheng Wu Gong Cheng Xue Bao. [Construction of recombinant human nerve growth factor (rh-β-NGF) eukaryotic vector and its expression in HEK293 cells]

[5] Xiang-Zhen Yan, et al. J Periodontol. A pilot study evaluating the effect of recombinant human bone morphogenetic protein-2 and recombinant human beta-nerve growth factor on the healing of Class III furcation defects in dogs

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Keywords: Recombinant Human Beta-NGF Protein (E. coli), β-NGF; NGFB; Beta-Nerve Growth Factor supplier, Cytokines and Growth Factors, inhibitors, activators

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