Human β-Nerve Growth Factor (β-NGF) is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity.
Recombinant Human Beta-NGF is produced by E.coli expression system and expressed the target gene encoding Ser122-Ala241.
Measured by the cell proliferation assay using TF‑1 human erythroleukemic cells, the ED50 is 0.03-0.3 ng/ml.
Accession: P01138
APMol Mass: 14 KDa, reducing conditions
Endotoxin < 0.01 EU/µg
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
| Form | Lyophilized powder |
| Solubility (25°C) | Dissolve the lyophilized protein in distilled water. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at ≤ -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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