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Recombinant Enhanced Green Fluorecence Protein (E. coli)

Cat. No. M22505
Recombinant Enhanced Green Fluorecence Protein (E. coli) Structure


Size Price Availability Quantity
10ug USD 200  USD200 In stock
500ug USD 2200  USD2200 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 1 EU/µg
  • COA
  • MSDS
Biological Activity

Recombinant Enhanced Green fluorescent protein (EGFP) is a 26.9 kDa protein (composed of 239 a.a. residues) that shows green fluorescence in short-wave light (blue to ultraviolet). Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37℃, respectively. 

Accession: P42212 

AA Sequence: 


Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Chemical Information
Solubility (25°C) Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Storage Stored at ≤ -20°C, stable for one year after receipt
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Jian Ouyang, et al. Nature. RNA transcripts stimulate homologous recombination by forming DR-loops

[2] Daichi Kamiyama, et al. Nat Commun. Versatile protein tagging in cells with split fluorescent protein

[3] Kristen M Kwan, et al. Dev Dyn. The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs

[4] Nicole Maria Seibel, et al. Anal Biochem. Nuclear localization of enhanced green fluorescent protein homomultimers

[5] S Hammoumi, et al. Arch Virol. Characterization of a recombinant encephalomyocarditis virus expressing the enhanced green fluorescent protein

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Keywords: Recombinant Enhanced Green Fluorecence Protein (E. coli), reGFP; EGFP supplier, Recombinant Proteins, inhibitors, activators

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