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Recombinant Enhanced Green Fluorecence Protein (E. coli)

Cat. No. M22505
Recombinant Enhanced Green Fluorecence Protein (E. coli) Structure
Synonym:

reGFP; EGFP

Size Price Availability Quantity
10ug USD 200  USD200 In stock
500ug USD 2200  USD2200 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 1 EU/µg
  • COA
  • MSDS
Biological Activity

Recombinant Enhanced Green fluorescent protein (EGFP) is a 26.9 kDa protein (composed of 239 a.a. residues) that shows green fluorescence in short-wave light (blue to ultraviolet). Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37℃, respectively. 


Accession: P42212 

AA Sequence: 

MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK 

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Chemical Information
Solubility (25°C) Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Storage Stored at ≤ -20°C, stable for one year after receipt
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Jian Ouyang, et al. Nature. RNA transcripts stimulate homologous recombination by forming DR-loops

[2] Daichi Kamiyama, et al. Nat Commun. Versatile protein tagging in cells with split fluorescent protein

[3] Kristen M Kwan, et al. Dev Dyn. The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs

[4] Nicole Maria Seibel, et al. Anal Biochem. Nuclear localization of enhanced green fluorescent protein homomultimers

[5] S Hammoumi, et al. Arch Virol. Characterization of a recombinant encephalomyocarditis virus expressing the enhanced green fluorescent protein

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Keywords: Recombinant Enhanced Green Fluorecence Protein (E. coli), reGFP; EGFP supplier, Recombinant Proteins, inhibitors, activators


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