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Phosphatidylcholine is a major constituent of cell membranes and pulmonary surfactant, and is more commonly found in the exoplasmic or outer leaflet of a cell membrane. Phosphatidylcholine also plays a role in membrane-mediated cell signaling and PCTP activation of other enzymes.
Journal of Army Medical University. 2024;46(5): 410-418.
Affinity test of 18 traditional Chinese medicine polysaccharides with Siglec-9 and their anti-inflammatory effects
Phosphatidylcholine purchased from AbMole
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Source | Nat Commun (2017). Figure 5. Phosphatidylcholine |
| Method | Immunocytochemistry | |
| Cell Lines | HeLa cells | |
| Concentrations | 5 mg/mL | |
| Incubation Time | 30 min | |
| Results | Within 30 min after adding liposomes, whereas PC fluorescent signal inside the cells was weak (Fig. 5i), PS-positive vesicles were readily detected inside WT MEF. |
| Cell Experiment | |
|---|---|
| Cell lines | Chang liver cells |
| Preparation method | Chang liver cells were plated in a 96-well dish (six replicates per sample), then treated with ethanol for 10 h, simultaneously and after the different concentrations of phosphatidylcholine (50, 75, and 100 nmol/L) were incubated for 24 h. 50 ll of MTT (5 mg/ml in PBS) was added to each well. |
| Concentrations | 50, 75, and 100 nmol/L |
| Incubation time | 24 h |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 758.06 |
| Formula | C42H80NO8P |
| CAS Number | 8002-43-5 |
| Solubility (25°C) | Ethanol ≥ 8 mg/mL |
| Storage | 2-8°C, dry, protect from light, sealed |
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