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Ortho-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/T-rich DNA strand. Although it binds to all nucleic acids, the A/T-rich double strand DNA significantly enhances fluorescence intensity. Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
| Molecular Weight | 534.39 |
| Formula | C25H23IN6 |
| CAS Number | 158013-41-3 |
| Solubility (25°C) | DMSO 30 mg/mL |
| Storage | 4°C, protect from light |
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