Oligomycin A inhibits membrane bound mitochondrial ATP synthase (ATPase), proton channel (pump, FO subunit) blockers to the cell membrane. Oligomycin A also inhibits oxidative phosphorylation and the electron transport chain. Oligomycin A decreased cytoplasmic ATP contents and disassembled metaphase II oocyte spindles when introduced to mouse oocytes. Oligomycin A is used to prevent state 3 (phosphorylating) respiration in oxidative phosphorylation research.
Mitochondrion. 2024 Feb 24;76:101856.
Miro1 improves the exogenous engraftment efficiency and therapeutic potential of mitochondria transfer using Wharton's jelly mesenchymal stem cells
Oligomycin A purchased from AbMole
Biol Reprod. 2021 May 7;104(5):1114-1125.
Mitochondrial transfer from induced pluripotent stem cells rescues developmental potential of in vitro fertilized embryos from aging females
Oligomycin A purchased from AbMole
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Source | Biol Reprod (2021 May 7). Figure 1. oligomycin (Abmole Bioscience, Houston, TX, USA) |
| Method | ATP assay | |
| Cell Lines | oocytes or embryos | |
| Concentrations | 1 μM | |
| Incubation Time | 20 min | |
| Results | By treating aging and young oocytes with specific inhibitors of OXPHOS (oligomycin) or glycolysis (sodium oxamate), we blocked ATP production by OXPHOS and glycolysis respectively, and found ATP production is young oocytes is predominantly dependent on OXPHOS; by contrast, aging oocytes displayed a decreased dependence on ATP production by OXPHOS, suggesting the impaired mitochondrial functions. In addition, we found this defect would be maintained by 2-cell stage |
| Cell Experiment | |
|---|---|
| Cell lines | H1229 cell |
| Preparation method | ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism. |
| Concentrations | 100 ng/ml |
| Incubation time | 1 h |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 791.06 |
| Formula | C45H74O11 |
| CAS Number | 579-13-5 |
| Solubility (25°C) | DMSO |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related ATPase Products |
|---|
| Transdermal Peptide Disulfide
Transdermal Peptide Disulfide (TD 1 Disulfide(peptide)) is a 11-amino acid peptide, binds toNa+/K+-ATPase beta-subunit (ATP1B1), and mainly interacts with the C-terminus of ATP1B1. |
| SPAI-1
SPAI-1 is a specific inhibitor for monovalent cation transporting ATPases. |
| Prilocaine acetate
Prilocaine acetate, an amino amide, is a Na/K-ATPase inhibitor. |
| Chamigrenol
Chamigrenol is a Na+/K+-ATPase inhibitor with an IC50 value of 15.9 μg/mL. |
| ZW290
ZW290 is a compound to activate brown adipose tissue (BAT) thermogenic function. |
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