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Nitroreductase is a flavoprotein and is encoded by the NfsB gene. Nitroreductase has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes. Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer research and improves sensitization of CB1954. Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical. Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.
Enzymatic Activity Unit Definition: One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.
| Form | Lyophilized powder |
| Storage | -20°C |
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