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N-Methylisatoic anhydride (NMIA) is a 2'-OH selective acylation agent of RNAs, and is widely used for resolving secondary RNA structures using the SHAPE (Selective 2'-Hydroxyl Acylation Analyzed by Primer Extension) technology.
| Molecular Weight | 177.16 |
| Formula | C9H7NO3 |
| CAS Number | 10328-92-4 |
| Form | Solid |
| Solubility (25°C) | DMSO 100 mg/mL (ultrasonic) |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
[1] Jaime F Martínez Suárez, et al. J Org Chem. Electrosynthesis of N-Methylisatin
[3] M A Faner, et al. Methods. Identifying and characterizing Hfq-RNA interactions
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