Free shipping on all orders over $ 500

Ingenol Mebutate

Cat. No. M5030
Ingenol Mebutate Structure
Synonym:

Ingenol 3-angelate; PEP005

Size Price Availability Quantity
5mg USD 225  USD250 In stock
10mg USD 369  USD410 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
  • Current batch:
  • Purity >98%
  • COA
  • MSDS
Biological Activity

Ingenol mebutate gel applied topically for 2 to 3 days is effective for field treatment of actinic keratoses.Ingenol mebutate is a Cell Death Inducer. The physiologic effect of ingenol mebutate is by means of Increased Cellular Death.

Customer Product Validations & Biological Datas
Source Mol Cancer Ther (2015). Figure 4. Ingenol Mebutate
Method qPCR
Cell Lines SCC cells
Concentrations 100 nmol/L
Incubation Time 24 h
Results In addition, we used either the PKC inhibitor AEB071 or a knockdown of PKCd, either alone or in combination with ingenol mebutate and analyzed the expression of ERK target genes. Either inhibition of PKC or knockdown of PKCd could prevent the upregulation of ERK target gene expression after ingenol mebutate treatment
Source Mol Cancer Ther (2015). Figure 3. Ingenol Mebutate
Method Western blotting
Cell Lines SCC13 cells
Concentrations 100 nmol/L
Incubation Time 24 h
Results Similar to our findings with the PKC inhibitor, we detected a rescue of cell viability upon ingenol mebutate treatment when PKCd was knocked down. A knockdown PKCa followed by ingenol mebutate treatment led only to a partial rescue, while PKCd knockdown could rescue the ingenol mebutate–treated cells completely
Protocol
Cell Experiment
Cell lines T7 cell
Preparation method Tumours were treated topically (on days 4 and 5 post tumour inoculation) with 30 μl of 0.25 % (w/v) ingenol mebutate or placebo gel (supplied by LEO Pharma A/S) using a positive displacement pipette, with the gel then spread over the tumour site.
Concentrations 30 μl of 0.25 % (w/v)
Incubation time 48 h
Animal Experiment
Animal models BALB/c nu−/nu− mice
Formulation isopropanol-based gel
Dosages 18 μg/kg
Administration i.v.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 430.53
Formula C25H34O6
CAS Number 75567-37-2
Purity >98%
Solubility 41 mg/ml in CHCl3
Storage at -20°C
References

Effective treatment of squamous cell carcinomas with ingenol mebutate gel in immunologically intact SKH1 mice.
Cozzi SJ, et al. Arch Dermatol Res. 2013 Jan;305(1):79-83. PMID: 22871992.

Ingenol mebutate gel for actinic keratosis.
Lebwohl M,et.al. N Engl J Med. 2012 Mar 15;1010-9. PMID: 22417254.

Related PKC Products
Delcasertib

Delcasertib (KAI-9803) is a potent and selective δ-protein kinase C (δPKC) inhibitor, it could ameliorate injury associated with ischemia and reperfusion in animal models of acute myocardial infarction (MI).

VTX-27

VTX-27 is a novel potent and selective PKCθ inhibitor, with Kis of 0.08 nM and 16 nM for PKC θ and PKC δ.

PKCβ inhibitor

PKCβ inhibitor is a potent and selective inhibitor of PKCβ isozymes (IC50 = 5 nM and 21 nM for human PKCβII and βI).

Chelerythrine Chloride

Chelerythrine is potent, cell-permeable inhibitor of protein kinase C (IC50 = 660 nM); competitive with respect to the phosphate acceptor and non-competitive with respect to ATP.

1-Naphthyl PP1

1-Naphthyl PP1(1-NA-PP 1) is a selective inhibitor of src family kinases v-Src and c-Fyn as well as the tyrosine kinase c-Abl (IC50 values are 1.0, 0.6, 0.6, 18 and 22 μM for v-Src, c-Fyn, c-Abl, CDK2 and CAMK II respectively).

  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Ingenol Mebutate, Ingenol 3-angelate; PEP005 supplier, PKC, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.