Species: Human
Purity: SDS-PAGE analysis, > 95%, HIGH performance liquid chromatography analysis, > 95%
Endotoxin level: protein < 0.2 EU/μg by gel coagulation
Apparent molecular weight: ~7.6 kDa, sdS-PAGE under reduction conditions
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles
Bioactivity: Insulin-like Growth Factor II (IGF-II) is a 7 kDa single-chain polypeptide with a high degree of homology to insulin. During in-body circulation, IGF-II is complex with IGF binding proteins (IGFBP), which act as a circulating reservoir to transport IGF-II and extend its half-life. The receptor of IGF-II (IGFRs) is a transmembrane tyrosine receptor, which is a heterotetramer composed of two α- and two β- subunits. IGFRs function through intracellular signaling molecules such as IRS, SHC, and PI3K. Igf-ii can promote breast cancer cell proliferation, inhibit death and stimulate transformation.
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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