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Ibuprofen

Cat. No. M3359
Ibuprofen Structure
Size Price Availability Quantity
500mg USD 40  USD40 In stock
1g USD 50  USD50 In stock
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Quality Control & Documentation
Biological Activity

Ibuprofen (Advil) is a non-selective COX inhibitor with an IC50 of 0.33 mM. It is known to have an antiplatelet effect, though Ibuprofen (Advil) is relatively mild and short-lived when compared with that of aspirin or other better-known antiplatelet agents. Ibuprofen, indomethacin, and meclofenamate were potent inhibitors of the microsomal cyclooxygenase prepared from feline lung with IC50 of 0.33 mM, 29 μM, and 4.7 μM, respectively.

Product Citations
Customer Product Validations & Biological Datas
Source Front Pharmacol (2021). Figure 5. ibuprofen (Abmole Bioscience, USA)
Method Oxytocin-Induced Dysmenorrhea in Mice
Cell Lines Female ICR mice
Concentrations 0.54 g/kg
Incubation Time seven days (once a day)
Results As shown in Figure 5, GZFLC significantly inhibited the oxytocininduced writhing response. The inhibition percentages of writhing times were 43.3% and 52.8% after oral administration of GZFLC (0.54 g/kg and 1.08 g/kg) respectively while 85.5% after oral administration of ibuprofen. The numbers of writhing times at GZFLC groups (0.54 g/kg and 1.08 g/kg) and ibuprofen group (0.1 g/kg) were 18.5 ± 4.5, 15.4 ± 4.6 and 5.9 ± 2.1 respectively, significantly lower than that of the model group (32.6 ± 8.5).
Protocol (for reference only)
Cell Experiment
Cell lines Bladder epithelial cell line T24, RT-4 transitional cell papilloma bladder cell line, 5637 primary carcinoma bladder cell line, HCT-116, MDAMB231, MCF7, HEK293, A549, SKOV3 and DU145
Preparation method Incubating each cell line with Ibuprofe of various concentrations for 48 hours. Estimating cell survival by the MTT assay, and cell death is determined by Hoechst staining used to distinguish between intact cell nuclei and fragmented nuclei undergoing cell death. Using western blotting for detection of the p75NTR protein to lysing and analyzing the cells .
Concentrations Dissolved in DMSO at a concentration of 200 mM for making the stock solution, final concentration ~2 mM
Incubation time 48 hours
Animal Experiment
Animal models Female Sprague-Dawley rats with joint inflammation induced by high-repetition and high-force (HRHF) tasks
Formulation Dissolved in DMSO and diluted in saline
Dosages 45 mg/kg
Administration Taken orally every day
Chemical Information
Molecular Weight 206.28
Formula C13H18O2
CAS Number 15687-27-1
Solubility (25°C) DMSO 40 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Min-Woo Ha, et al. Recent Advances in the Synthesis of Ibuprofen and Naproxen

[2] Frideriki Poutoglidou, et al. Ibuprofen and COVID-19 disease: separating the myths from facts

[3] Parth N Shah, et al. Antimicrobial Activity of Ibuprofen against Cystic Fibrosis-Associated Gram-Negative Pathogens

[4] Thomas Legg, et al. Single- and Multiple-Dose Pharmacokinetics of Immediate-Release/Extended-Release Ibuprofen Tablets

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  Catalog
Abmole Inhibitor Catalog




Keywords: Ibuprofen supplier, COX, inhibitors, activators


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