All AbMole products are for research use only, cannot be used for human consumption.
Ibuprofen (Advil) is a non-selective COX inhibitor with an IC50 of 0.33 mM. It is known to have an antiplatelet effect, though Ibuprofen (Advil) is relatively mild and short-lived when compared with that of aspirin or other better-known antiplatelet agents. Ibuprofen, indomethacin, and meclofenamate were potent inhibitors of the microsomal cyclooxygenase prepared from feline lung with IC50 of 0.33 mM, 29 μM, and 4.7 μM, respectively.
Front Pharmacol. 2021 Mar 3;12:613104.
Deciphering the Pharmacological Mechanisms of Guizhi-Fuling Capsule on Primary Dysmenorrhea Through Network Pharmacology
Ibuprofen purchased from AbMole
 |
Source | Front Pharmacol (2021). Figure 5. ibuprofen (Abmole Bioscience, USA) |
| Method | Oxytocin-Induced Dysmenorrhea in Mice | |
| Cell Lines | Female ICR mice | |
| Concentrations | 0.54 g/kg | |
| Incubation Time | seven days (once a day) | |
| Results | As shown in Figure 5, GZFLC significantly inhibited the oxytocininduced writhing response. The inhibition percentages of writhing times were 43.3% and 52.8% after oral administration of GZFLC (0.54 g/kg and 1.08 g/kg) respectively while 85.5% after oral administration of ibuprofen. The numbers of writhing times at GZFLC groups (0.54 g/kg and 1.08 g/kg) and ibuprofen group (0.1 g/kg) were 18.5 ± 4.5, 15.4 ± 4.6 and 5.9 ± 2.1 respectively, significantly lower than that of the model group (32.6 ± 8.5). |
| Cell Experiment | |
|---|---|
| Cell lines | Bladder epithelial cell line T24, RT-4 transitional cell papilloma bladder cell line, 5637 primary carcinoma bladder cell line, HCT-116, MDAMB231, MCF7, HEK293, A549, SKOV3 and DU145 |
| Preparation method | Incubating each cell line with Ibuprofe of various concentrations for 48 hours. Estimating cell survival by the MTT assay, and cell death is determined by Hoechst staining used to distinguish between intact cell nuclei and fragmented nuclei undergoing cell death. Using western blotting for detection of the p75NTR protein to lysing and analyzing the cells . |
| Concentrations | Dissolved in DMSO at a concentration of 200 mM for making the stock solution, final concentration ~2 mM |
| Incubation time | 48 hours |
| Animal Experiment | |
|---|---|
| Animal models | Female Sprague-Dawley rats with joint inflammation induced by high-repetition and high-force (HRHF) tasks |
| Formulation | Dissolved in DMSO and diluted in saline |
| Dosages | 45 mg/kg |
| Administration | Taken orally every day |
| Molecular Weight | 206.28 |
| Formula | C13H18O2 |
| CAS Number | 15687-27-1 |
| Solubility (25°C) | DMSO 40 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
[1] Min-Woo Ha, et al. Recent Advances in the Synthesis of Ibuprofen and Naproxen
[2] Frideriki Poutoglidou, et al. Ibuprofen and COVID-19 disease: separating the myths from facts
| Related COX Products |
|---|
| Sudoxicam
Sudoxicam is a reversible and orally active COX antagonist and a non-steroidal anti-inflammatory agent from the enol-carboxamide class. |
| Metyrosine
Metyrosine is a selective tyrosine hydroxylase enzyme inhibitor. Metyrosine significantly inhibits high COX-2 activity. Metyrosine exerts anti-inflammatory and anti-ulcerative effects. |
| PPOH
PPOH, a fatty acid derivative, is a selective cyclooxygenase (COX) inhibitor that inhibits arachidonic acid cyclooxygenase activity in renal cortical microsomes. |
| Tenidap
Tenidap, a non-steroidal anti-inflammatory drug, is a selective COX-1 inhibitor, with IC50 values of 0.03 μM and 1.2 μM for COX-1 and COX-2, respectively. |
| COX-2-IN-6
COX-2-IN-6 is an orally active, gut-restricted and selective cyclooxygenase-2 (COX-2) inhibitor for colorectal Chemoprevention of cancer. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
