All AbMole products are for research use only, cannot be used for human consumption.
Ibuprofen (Advil) is a non-selective COX inhibitor with an IC50 of 0.33 mM. It is known to have an antiplatelet effect, though Ibuprofen (Advil) is relatively mild and short-lived when compared with that of aspirin or other better-known antiplatelet agents. Ibuprofen, indomethacin, and meclofenamate were potent inhibitors of the microsomal cyclooxygenase prepared from feline lung with IC50 of 0.33 mM, 29 μM, and 4.7 μM, respectively.
Front Pharmacol. 2021 Mar 3;12:613104.
Deciphering the Pharmacological Mechanisms of Guizhi-Fuling Capsule on Primary Dysmenorrhea Through Network Pharmacology
Ibuprofen purchased from AbMole
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Source | Front Pharmacol (2021). Figure 5. ibuprofen (Abmole Bioscience, USA) |
| Method | Oxytocin-Induced Dysmenorrhea in Mice | |
| Cell Lines | Female ICR mice | |
| Concentrations | 0.54 g/kg | |
| Incubation Time | seven days (once a day) | |
| Results | As shown in Figure 5, GZFLC significantly inhibited the oxytocininduced writhing response. The inhibition percentages of writhing times were 43.3% and 52.8% after oral administration of GZFLC (0.54 g/kg and 1.08 g/kg) respectively while 85.5% after oral administration of ibuprofen. The numbers of writhing times at GZFLC groups (0.54 g/kg and 1.08 g/kg) and ibuprofen group (0.1 g/kg) were 18.5 ± 4.5, 15.4 ± 4.6 and 5.9 ± 2.1 respectively, significantly lower than that of the model group (32.6 ± 8.5). |
| Cell Experiment | |
|---|---|
| Cell lines | Bladder epithelial cell line T24, RT-4 transitional cell papilloma bladder cell line, 5637 primary carcinoma bladder cell line, HCT-116, MDAMB231, MCF7, HEK293, A549, SKOV3 and DU145 |
| Preparation method | Incubating each cell line with Ibuprofe of various concentrations for 48 hours. Estimating cell survival by the MTT assay, and cell death is determined by Hoechst staining used to distinguish between intact cell nuclei and fragmented nuclei undergoing cell death. Using western blotting for detection of the p75NTR protein to lysing and analyzing the cells . |
| Concentrations | Dissolved in DMSO at a concentration of 200 mM for making the stock solution, final concentration ~2 mM |
| Incubation time | 48 hours |
| Animal Experiment | |
|---|---|
| Animal models | Female Sprague-Dawley rats with joint inflammation induced by high-repetition and high-force (HRHF) tasks |
| Formulation | Dissolved in DMSO and diluted in saline |
| Dosages | 45 mg/kg |
| Administration | Taken orally every day |
| Molecular Weight | 206.28 |
| Formula | C13H18O2 |
| CAS Number | 15687-27-1 |
| Solubility (25°C) | DMSO 40 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
[1] Min-Woo Ha, et al. Recent Advances in the Synthesis of Ibuprofen and Naproxen
[2] Frideriki Poutoglidou, et al. Ibuprofen and COVID-19 disease: separating the myths from facts
| Related COX Products |
|---|
| Firocoxib
Firocoxib is a selective COX-2 inhibitor exhibiting high selectivity to inhibit COX-2. |
| Ampiroxicam
Ampiroxicam is a nonselective cyclooxygenase inhibitor uesd as anti-inflammatory compound. |
| Bufexamac
Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM. |
| Carprofen
Carprofen inhibits canine COX2 with IC50 of 0.03 mM. |
| Diclofenac Diethylamine
Diclofenac diethylamine is a nonsteroidal anti-inflammatory compound taken to reduce inflammation and as an analgesic reducing pain in certain conditions. |
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