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H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm). H2DCFDA can be used for the Bacterial reactive oxygen species (ROS) production assay.
ROS Measurements
For the detection of intracellular ROS level ,ROS-sensitive probe H2DCFDA is used. Adherent cells (ESCs, difESCs, eMSCs, HeLa, U118) are incubated with 5 µM staining solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. Lymphocytes, both control and PHA-activated, are resuspended in PBS, incubated with 5 µM of H2DCFDA in the dark for 30 min at 37°C, and immediately analyzed. Along with the H2DCFDA probe, if indicated, ROS-insensitive modification of the fluorescent dye DCFDA is used. The staining procedure is the same as for the H2DCFDA.
Mater Today Bio. 2025 Feb 03.
Nature-derived microneedles with metal-polyphenolic networks encapsulation for chronic soft tissue defects repair: Responding and remodeling the regenerative microenvironment
H2DCFDA purchased from AbMole
Science Advances. 2024 Aug 16;10(33):eado7249.
Magnetically attracting hydrogel reshapes iron metabolism for tissue repair
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J Colloid Interface Sci. 2024 Jun;663:810-824..
Amino acid-based metallo-supramolecular nanoassemblies capable of regulating cellular redox homeostasis for tumoricidal chemo-/photo-/catalytic combination therapy
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GeroScience. 2024 Dec 27.
Paroxetine promotes longevity via ser-7-dop-4-IIS axis in Caenorhabditis elegans
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Int Immunopharmacol. 2024 Sep 12;142(Pt A):113041.
Salidroside alleviates ferroptosis in FAC-induced Age-related macular degeneration models by activating Nrf2/SLC7A11/GPX4 axis
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Foods. 2024 Dec 14.
Chemometric Classification and Bioactivity Correlation of Black Instant Coffee and Coffee Bean Extract by Chlorogenic Acid Profiling
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Plant Biotechnol J. 2023 May;21(5):943-960.
The C2H2-type zinc finger transcription factor OSIC1 positively regulates stomatal closure under osmotic stress in poplar
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Materials Today Bio. 2023 Jun 19.
Melanin inspired microcapsules delivering immune metabolites for hepatic fibrosis management
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J Agric Food Chem. 2023 Nov 22;71(46):18059-18073.
Transcriptome Analysis of GmPUB20A Overexpressing and RNA-Interferencing Transgenic Hairy Roots Reveals Underlying Negative Role in Soybean Resistance to Cyst Nematode
H2DCFDA purchased from AbMole
Int Immunopharmacol. 2023 Sep 21;124(Pt B):110963.
BNTA attenuates temporomandibular joint osteoarthritis progression by directly targeting ALDH3A1: An in vivo and in vitro study
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Burns Trauma. 2023 Oct 31;11:tkad030.
S100 calcium-binding protein A9 promotes skin regeneration through toll-like receptor 4 during tissue expansion
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Environ Toxicol. 2023 Jul 29.
Wogonin alleviates BaP-induced DNA damage and oxidative stress in human airway epithelial cells by dual inhibiting CYP1A1 activity and expression
H2DCFDA purchased from AbMole
Biochem Biophys Res Commun. 2023 Oct 5;682:163-173.
Experimental and theoretical studies on inhibition against tyrosinase activity and melanin biosynthesis by antioxidant ergothioneine
H2DCFDA purchased from AbMole
J Cosmet Dermatol. 2023 Jul 10.
Potent pigmentation inhibitory activity of incensole‐enriched frankincense volatile oil‐identification, efficacy and mechanism
H2DCFDA purchased from AbMole
Stem Cell Rev Rep. 2022 Aug;18(6):2088-2103.
Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Skin Aging of Nude Mice Through Autophagy-Mediated Anti-Senescent Mechanism
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Parasit Vectors. 2022 Aug 1;15(1):274.
Neospora caninum infection induced mitochondrial dysfunction in caprine endometrial epithelial cells via downregulating SIRT1
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Comp Biochem Physiol C Toxicol Pharmacol. 2021 Apr;242:108938.
Clove essential oil confers antioxidant activity and lifespan extension in C. elegans via the DAF-16/FOXO transcription factor
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Ren Fail. 2021 Dec;43(1):900-910.
MMP-9-mediated regulation of hypoxia-reperfusion injury-related neutrophil inflammation in an in vitro proximal tubular cell model
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Mediators Inflamm. 2020 Jun 6;2020:4321912.
CD137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating NF- κ B Pathway
H2DCFDA purchased from AbMole
Anat Rec (Hoboken). 2020 Jan 21.
Resibufogenin inhibited colorectal cancer cell growth and tumorigenesis through triggering ferroptosis and ROS production mediated by GPX4 inactivation
H2DCFDA purchased from AbMole
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Source | Comp Biochem Physiol C Toxicol Pharmacol (2021 Apr), Figure 3, H2DCFDA (Abmole Bioscience) |
| Method | measure the ROS levels | |
| Cell Lines | Each C. elegans strain | |
| Concentrations | 50 μM | |
| Incubation Time | 1 h | |
| Results | The results showed that CEO exposure made no significant difference to the GSH amount in each worm, however, the lipid peroxidation degree decreased due to the reduced ROS levels. |
| Molecular Weight | 487.29 |
| Formula | C24H16Cl2O7 |
| CAS Number | 4091-99-0 |
| Solubility (25°C) | DMSO: ≥ 90 mg/mL |
| Storage | -20°C, protect from light |
[1] Karyne Rangel, et al. Microorganisms. Detrimental Effect of Ozone on Pathogenic Bacteria
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