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4-Methylumbelliferyl-α-D-Glucopyranoside is a fluorescent substrate for α-glucosidase, which releases the fluorescent moiety 4-methylumbelliferyl (4-MU) upon cleavage. 4-MU has pH-dependent fluorescence excitation activity, with excitation wavelengths of 320 nm at low pH (1.97-6.72) and 360 nm at high pH (7.12-10.3), respectively. The emission wavelength of 4-Methylumbelliferyl-α-D-Glucopyranoside increases with decreasing pH, ranging from 445-455 nm. 4-Methylumbelliferyl-α-D-Glucopyranoside can be used as a biomarker for Fabry and Pompe diseases to quantify α-glucosidase activity in infant blood spot samples.
| Molecular Weight | 338.31 |
| Formula | C16H18O8 |
| CAS Number | 17833-43-1 |
| Form | Solid |
| Solubility (25°C) | DMSO 20 mg/mL |
| Storage | 4°C, sealed |
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