FGF-basic, Rat（E. coli）

Biological Activity

Expression system: E. coli
Purity: SDS-PAGE > 95%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: ~16.4 kDa, reduced by SDS-PAGE.
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.

Biological Activity: Basic fibroblast growth factor (FGF-BASIC)（E. coli）, also known as FGF-2, is a multipotent cytokine and one of the prototype members of the heparin-binding FGF family. Like other MEMBERS of the FGF family, alkaline FGF has a β trilobular structure. In vivo, BASIC fibroblast factor is produced by a variety of cells, including myocardial microbiota, fibroblasts, and vascular cells. BFGF regulates a variety of processes, including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation, and wound healing.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information

References

[1] Yong Li, et al. J Cardiovasc Pharmacol. Effect of NRG-1/ErbB signaling intervention on the differentiation of bone marrow stromal cells into sinus node-like cells

[2] F Marn, et al. J Pineal Res. Immunocytochemical localization of basic fibroblast growth factor in the rat pineal gland

[3] A U Dignass, et al. Gastroenterology. Fibroblast growth factors modulate intestinal epithelial cell growth and migration

[4] H Iwata, et al. Brain Res. Localization of basic FGF-like immunoreactivity in the hypothalamo-hypophyseal neuroendocrine axis

[5] J W Moore, et al. Development. The mRNAs encoding acidic FGF, basic FGF and FGF receptor are coordinately downregulated during myogenic differentiation