Expression system: P. pastoris
Purity: SDS-PAGE > 97%
Endotoxin: Protein < 1 EU/μg was determined by gel coagulation
Theoretical molecular weight: 20.2 kDa
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: Endostatin (P. pastoris) is a cleaved product of the XVIII carboxy-terminal domain of collagen. As an anti-angiogenic cytokine, it is expressed in various organs, especially in liver, lung and kidney at high levels. Endostatin inhibits angiogenesis by blocking the angiogenic activity of VEGF and FGF. In vitro studies have shown that endostatin prevents endothelial cell proliferation and the formation of new blood vessels. In animal studies, endostatin inhibits angiogenesis and growth in primary and secondary tumor metastases.
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[4] Priya Nijhawan, et al. Curr Rheumatol Rev. The Role of Endostatin in Rheumatoid Arthritis
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