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Enalaprilat Dihydrate

Cat. No. M3426
Enalaprilat Dihydrate Structure
Size Price Availability Quantity
50mg USD 60  USD60 In stock
100mg USD 100  USD100 In stock
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Quality Control & Documentation
Biological Activity

Enalaprilat dihydrate is the first dicarboxylate-containing ACE inhibitor with an IC50 of 4 nM. It was developed partly to overcome these limitations of captopril. The sulfhydryl-moiety was replaced by a carboxylate-moiety, but additional modifications were required in its structure-based design to achieve a similar potency to captopril.

Product Citations
Protocol (for reference only)
Cell Experiment
Cell lines Rat cardiac fibroblasts cell lines
Preparation method After 24 hours incubation in serum-free medium (DMEM), stimulating cells with IGF-I (1-100 nM) and coincubating with Enalaprilat (1 nM-10 μM) for 24 hours. Using a colorimetric immunoassay during the last 4 hours of the 24 hours incubation period to assesse cellular proliferation by 5-bromo-2'-deoxyuridine (BrdU) incorporation . Mearsuring the extinctions at 450 nm in an ELISA plate reader. All values consist of an n=9.
Concentrations Dissolved in DMSO, final concentrations 1 nM - 10 μM
Incubation time 24 hours
Animal Experiment
Animal models Male Sprague–Dawley rats
Formulation Dissolved in a vehicle solution (1 mg in 950 μL of phosphate buffered saline and 50 μL 1M Na2CO3).
Dosages 1 mg/kg
Administration I.V. bolus
Chemical Information
Molecular Weight 348.4
Formula C18H24N2O5.2H2O
CAS Number 84680-54-6
Solubility (25°C) DMSO 60 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Gabriel R Estrela, et al. Front Mol Biosci. Angiotensin-Converting Enzyme Inhibitor Protects Against Cisplatin Nephrotoxicity by Modulating Kinin B1 Receptor Expression and Aminopeptidase P Activity in Mice

[2] Cindy Gueguen, et al. Hypertens Res. Renal nerves contribute to hypertension in Schlager BPH/2J mice

[3] Kristy L Jackson, et al. Hypertension. A novel interaction between sympathetic overactivity and aberrant regulation of renin by miR-181a in BPH/2J genetically hypertensive mice

[4] T Yamasaki, et al. No To Shinkei. Effect of enalaprilat, an angiotensin converting enzyme inhibitor, on the membrane potential of cultured neuroblastoma-glioma hybrid NG108-15 cells

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Keywords: Enalaprilat Dihydrate supplier, ACE, inhibitors, activators

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