Free shipping on all orders over $ 500

Enalaprilat Dihydrate

Cat. No. M3426
Enalaprilat Dihydrate Structure
Size Price Availability Quantity
50mg USD 60  USD60 In stock
100mg USD 100  USD100 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
Biological Activity

Enalaprilat dihydrate is the first dicarboxylate-containing ACE inhibitor with an IC50 of 4 nM. It was developed partly to overcome these limitations of captopril. The sulfhydryl-moiety was replaced by a carboxylate-moiety, but additional modifications were required in its structure-based design to achieve a similar potency to captopril.

Product Citations
Protocol (for reference only)
Cell Experiment
Cell lines Rat cardiac fibroblasts cell lines
Preparation method After 24 hours incubation in serum-free medium (DMEM), stimulating cells with IGF-I (1-100 nM) and coincubating with Enalaprilat (1 nM-10 μM) for 24 hours. Using a colorimetric immunoassay during the last 4 hours of the 24 hours incubation period to assesse cellular proliferation by 5-bromo-2'-deoxyuridine (BrdU) incorporation . Mearsuring the extinctions at 450 nm in an ELISA plate reader. All values consist of an n=9.
Concentrations Dissolved in DMSO, final concentrations 1 nM - 10 μM
Incubation time 24 hours
Animal Experiment
Animal models Male Sprague–Dawley rats
Formulation Dissolved in a vehicle solution (1 mg in 950 μL of phosphate buffered saline and 50 μL 1M Na2CO3).
Dosages 1 mg/kg
Administration I.V. bolus
Chemical Information
Molecular Weight 348.4
Formula C18H24N2O5.2H2O
CAS Number 84680-54-6
Solubility (25°C) DMSO 60 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Gabriel R Estrela, et al. Front Mol Biosci. Angiotensin-Converting Enzyme Inhibitor Protects Against Cisplatin Nephrotoxicity by Modulating Kinin B1 Receptor Expression and Aminopeptidase P Activity in Mice

[2] Cindy Gueguen, et al. Hypertens Res. Renal nerves contribute to hypertension in Schlager BPH/2J mice

[3] Kristy L Jackson, et al. Hypertension. A novel interaction between sympathetic overactivity and aberrant regulation of renin by miR-181a in BPH/2J genetically hypertensive mice

[4] T Yamasaki, et al. No To Shinkei. Effect of enalaprilat, an angiotensin converting enzyme inhibitor, on the membrane potential of cultured neuroblastoma-glioma hybrid NG108-15 cells

Related ACE Products
Abz-Ser-Pro-3-nitro-Tyr

Abz-Ser-Pro-3-nitro-Tyr is the substrate of ACE2 (angiotensin-converting enzyme-2).

Temocaprilat

Temocaprilat (Temocapril diacid) is an inhibitor of angiotensin-converting enzyme (ACE).

FA-Phe-Phe

FA-Phe-Phe is a furylacryloyl (fa)-amino acid derivative, targeting to Angiotensin-converting Enzyme (ACE).

Garcinone B

Garcinone B, a xanthone derivative, is a nature product that could be isolated from the pericarp of Mangosteen.

Spirapril hydrochloride

Spirapril (SCH 33844) hydrochloride is a potent angiotensin converting enzyme (ACE) inhibitor with antihypertensive activity. Spirapril competitively binds to ACE and prevents the conversion of angiotensin I to angiotensin II. Spirapril is an orally active proagent of Spiraprilat and can be used for the research of hypertension, congestive heart failure.

  Catalog
Abmole Inhibitor Catalog




Keywords: Enalaprilat Dihydrate supplier, ACE, inhibitors, activators


Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.