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DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.
| Molecular Weight | 412.34 |
| Formula | C21H14F2N2O5 |
| CAS Number | 254109-20-1 |
| Solubility (25°C) | DMSO: ≥1 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
[2] Rachael Knoblauch, et al. Plasmonic enhancement of nitric oxide generation
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