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DAF-FM

Cat. No. M7748

All AbMole products are for research use only, cannot be used for human consumption.

DAF-FM Structure

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Biological Activity

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Chemical Information
Molecular Weight 412.34
Formula C21H14F2N2O5
CAS Number 254109-20-1
Solubility (25°C) DMSO: ≥1 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
References

[1] Marta Rodrguez-Ruiz, et al. Detection of Protein S-nitrosothiols (SNOs) in Plant Samples on Diaminofluorescein (DAF) Gels

[2] Rachael Knoblauch, et al. Plasmonic enhancement of nitric oxide generation

[3] Chris D St Laurent, et al. Measurement of nitric oxide in mast cells with the fluorescent indicator DAF-FM diacetate

[4] Shabnam M Namin, et al. Kinetic analysis of DAF-FM activation by NO: toward calibration of a NO-sensitive fluorescent dye

[5] Jan-Zhong Sheng, et al. DAF-FM (4-amino-5-methylamino-2',7'-difluorofluorescein) diacetate detects impairment of agonist-stimulated nitric oxide synthesis by elevated glucose in human vascular endothelial cells: reversal by vitamin C and L-sepiapterin

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Keywords: DAF-FM supplier, inhibitors, activators

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