Chelerythrine
 |
Source | Mol Cancer Res (2015). Figure 4. Chelerythrine |
| Method | Cell proliferation assay | |
| Cell Lines | 621-101 cells | |
| Concentrations | 5 μmol/L | |
| Incubation Time | 2 h | |
| Results | CI-1040 pretreatment did not abrogate the effects of chelerythrine chloride in 621-101 cells or Tsc2-null MEFs |
 |
Source | Mol Cancer Res (2015). Figure 3. Chelerythrine |
| Method | Western blot | |
| Cell Lines | Tsc2+/+ MEFs | |
| Concentrations | 2 μmol/L | |
| Incubation Time | 4 h | |
| Results | Chelerythrine chloride induced PARP cleavage in Tsc2-null MEFs but not Tsc2-expressing MEFs. |
| Cell Experiment | |
|---|---|
| Cell lines | SERCA |
| Preparation method | For the phosphorylation by [32P]Pi, 100 lgmL1 SRV protein in an E2-favoring reaction buffer (20 mM MES-pH 6.0-with 10 mM MgCl2, 2 mM EGTA, and the indicated chelerythrine concentrations) was incubated and 4 mM [32P]Pi were added. |
| Concentrations | 1, 10, 50 and 100 μM |
| Incubation time | 20–40 min, |
| Animal Experiment | |
|---|---|
| Animal models | BALB/c (H2d) mice |
| Formulation | |
| Dosages | 25, 50, 100, and 200 mg/kg |
| Administration | i.p. |
| Molecular Weight | 383.83 |
| Formula | C21H18ClNO4 |
| CAS Number | 34316-15-9 |
| Solubility (25°C) | DMSO 2 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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