BDNF, also known as brain-derived neurotrophic factor and abrineurin, is a neurotrophic factor belonging to the NGF - β family. It is highly expressed in the brain and moderately expressed in the heart, lungs, skeletal muscle, and placenta. BDNF transmits neurotrophic properties through its high-affinity receptor GP145 /trkB. It has been shown to be involved in the survival and differentiation of the central and peripheral nervous systems. Specifically, BDNF regulates synaptic transmission, axon growth and pathfinding, and dendrite growth and morphology.
Immobilized Human BDNF at 5 μg/ml (100 μl/well) can bind NGF R, Human-Biotin with a linear range of 0.06-4 μg/ml.
Accession: P23560
Apparent Molecular Weight: 12~14 kDa, reducing conditions
Endotoxin < 0.2 EU/μg
Lyophilized after extensive dialysis against PBS.
| Form | Lyophilized powder |
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS to 100 μg/ml. |
| Storage | Stable for up to 6 months at ≤ -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[3] Manju Sasi, et al. Pflugers Arch. Neurobiology of local and intercellular BDNF signaling
[4] Carl Bjrkholm, et al. Neuropharmacology. BDNF - a key transducer of antidepressant effects
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