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BCECF-AM is a cell membrane permeable compound, widely used as a fluorescent indicator for intracellular pH.
AMACS Appl Mater Interfaces. 2022 Feb 2;14(4):5033-5052.
Trojan-Horse Diameter-Reducible Nanotheranostics for Macroscopic/Microscopic Imaging-Monitored Chemo-Antiangiogenic Therapy
BCECF-AM purchased from AbMole
| Cell Experiment | |
|---|---|
| Cell lines | HC and CF neutrophils phagosomes of S. aureus |
| Preparation method | Phagosome pH Measurements The pH of phagosomes of HC and CF neutrophils containing 2′,7′-Bis(2-carboxyethyl)-5 (6)- carboxyfluorescein acetoxymethyl ester (BCECF-AM, 5 μM) labelled S. aureus were assessed. Pasteurized S. aureus (1x10^9 c.f.u) were pre-loaded with BCECF-AM dye (10 µM) for 30 min before removal of excess dye and opsonization with 1% (w/v) human IgG for 30 min. Neutrophils (2 x 10^7) suspended in PBSG pH 7.4 were rapidly stirred in a 37°C thermostatically controlled oxygen electrode chamber. BCECF-AM labelled S. aureus (1 x 10^8 c.f.u.) was added and aliquots removed at indicted time points up to 16 min. The intracellular pH measurements with BCECF were made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) with the dye excited at 490 nm versus the emission intensity at 440 nm and correlated to pH values using an established pH standard curve (range pH 6–8). |
| Concentrations | 10 µM |
| Incubation time | 30 min |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 808.69 |
| Formula | C39H36O19 |
| CAS Number | 117464-70-7 |
| Solubility (25°C) | DMSO |
| Storage | -20°C, dry, protect from light, sealed |
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